Objective : To investigate the effect of BUM aqua-acupuncture in treating the RA, the immunosis to logical analysis of LPS induced arthritis in mice to study this.
For 14th day after the injection of LPS & BUM injection, the distribution of fibroblast, collagen, CD54(ICAM-1), CD106(VCAM-1), IL-1β, IL-2 receptor, CDl lb(macrophage) were examined on synovial capsule of mice knee joint.
For 14th day after the injection of LPS & BUM injection, the distribucion of CD4(TH cell), CD8(TC cell), CD40(B cell) were examined on common iliac lymph node in mice.
Methods : The experimental model of arthritis was induced by injection of 300㎍/㎏ LPS in BALB/c mice weighing 30g.
The 100㎕ BUM aqua-acupuncture which compounded calculus bovis, fel ursi and moschus was injected into GB34 of mice every other day for 12 days.
For 3rd, 7th, 14th day after the injection of LPS, the neutrophil, lymphocyte and monocytc counts in WBC were measured using hemacytometer.
Results : The obstain results are summarized as follows ;
1. In sample group, the neutrophils counts were increased and the lympnocytes counts were decreased compared with control group.
2. The distribution of fibrosis & fibroblast on synovial membrane were decreased compared with control group.
3. The distribution of collagen fiber on synovial membrane were decreased compared´ with control group.
4. The distribution of CD54(ICAM-1) & CD106(VCAM-1) on synovial membrane were decreased compared with control group.
5. The distribution of IL-1β & IL-2 receptor on synovial membrane were decreased compared with control group.
6. The distribution of CDb(macrophage) on synovial membrane were decreased compared with control group.
7. The distribution of CD4(TH cell), CD8(TC cell) and CD40(B cell) in common iliac lymph nodes were decreased compared with control group.
Conclusions : BUM aqua-acupuncture stimulation decreased inflammatory responses LPS induced arthritis in mice.
|